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Journal: Frontiers in Immunology
Article Title: Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling
doi: 10.3389/fimmu.2026.1636331
Figure Lengend Snippet: Detection of protein expression and functional verification of CD36 and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
Article Snippet: Subsequently,
Techniques: Expressing, Functional Assay, Immunohistochemical staining, Staining, Migration
Journal: Frontiers in Immunology
Article Title: Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling
doi: 10.3389/fimmu.2026.1636331
Figure Lengend Snippet: Correlation analysis of CD36, KIT with TME and PA. (A) Detection of IFN-γ secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (B) Detection of TNF-α secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001.**** denotes P < 0.0001. (C) Detection of IL-10 secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors.** denotes P < 0.01. *** denotes P < 0.001. (D) Immunofluorescence staining results of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (E) Immunofluorescence staining results of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (F) Differential analysis of immunofluorescence optical density of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (G) Differential analysis of the ratio of nuclear to total immunofluorescence optical density of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors.**** denotes P < 0.0001.
Article Snippet: Subsequently,
Techniques: Immunofluorescence, Staining