Journal: Cells

Article Title: Imeglimin Inhibits Macrophage Foam Cell Formation and Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-Deficient Mice

doi: 10.3390/cells14070472

Figure Lengend Snippet: Effects of imeglimin on the expression of ABCA1, ABCG1, and CD36 proteins in ox-LDL/HG-treated THP1 macrophages. THP-1 macrophages were pretreated with imeglimin and then exposed to 100 μg/mL ox-LDL and 30 mM glucose. ( A ) Western blot analysis showing the expression of ABCA1, ABCG1, and CD36 proteins; ( B ) Relative protein expression levels of ABCA1, ABCG1, and CD36 (quantified using ImageJ). Results are expressed as the means ± SEMs of at least three independent experiments. * p < 0.05, ** p < 0.01. HG, high glucose; IME, imeglimin; oxLDL, oxidized lowdensity lipoproteins.

Article Snippet: Membranes were blocked using 5% bovine serum albumin at room temperature for 1 h, followed by overnight incubation at 4 °C with primary antibodies targeting CD36 (PA1-16813, 1:1000, Thermo Fisher Scientific), ABCA1 (NB400-105, 1:1000, Novus Biologicals, Littleton, CO, USA), ABCG1 (NB400-132, 1:1000, Novus Biologicals), AMPK (2532, 1:1000, Cell Signaling Technology), phosphorylated AMPK (pAMPK, 2535, 1:1000, Cell Signaling Technology), and β-actin (ab8227, 1:10,000, Abcam, Cambridge, UK).

Techniques: Expressing, Western Blot

Journal: Cells

Article Title: Imeglimin Inhibits Macrophage Foam Cell Formation and Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-Deficient Mice

doi: 10.3390/cells14070472

Figure Lengend Snippet: Effect of imeglimin on the expression of ABCA1, ABCG1, and CD36 in the presence of the AMPK inhibitor compound C. THP-1-derived macrophages were pretreated with compound C, incubated with 100 μM imeglimin for 24 h, and exposed to 100 μg/mL oxLDL and 30 mM glucose. ( A ) Western blot analysis showing the expression of total AMPK (tAMPK) and phosphorylated AMPK ( p -AMPK) proteins; ( B ) Relative protein expression levels of t-AMPK and pAMPK (quantified using ImageJ); ( C ) Western blot analysis showing the expression of ABCA1, ABCG1, and CD36 proteins; ( D ) Relative protein expression levels of ABCA1, ABCG1, and CD36 (quantified using ImageJ). Results are expressed as the means ± SEMs of at least three independent experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001. HG, high glucose; IME, imeglimin; ox-LDL, oxidized low-density lipoproteins.

Article Snippet: Membranes were blocked using 5% bovine serum albumin at room temperature for 1 h, followed by overnight incubation at 4 °C with primary antibodies targeting CD36 (PA1-16813, 1:1000, Thermo Fisher Scientific), ABCA1 (NB400-105, 1:1000, Novus Biologicals, Littleton, CO, USA), ABCG1 (NB400-132, 1:1000, Novus Biologicals), AMPK (2532, 1:1000, Cell Signaling Technology), phosphorylated AMPK (pAMPK, 2535, 1:1000, Cell Signaling Technology), and β-actin (ab8227, 1:10,000, Abcam, Cambridge, UK).

Techniques: Expressing, Derivative Assay, Incubation, Western Blot

Journal: Cells

Article Title: Imeglimin Inhibits Macrophage Foam Cell Formation and Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-Deficient Mice

doi: 10.3390/cells14070472

Figure Lengend Snippet: Effects of imeglimin on AMPK activity and ABCA1, ABCG1, and CD36 protein expression in diabetic ApoE -/- mice. ( A ) Western blot analysis showing the expression of pAMPK, tAMPK, ABCA1, ABCG1, and CD36 in the liver; ( B ) Relative protein expression levels of pAMPK, tAMPK, ABCA1, ABCG1, and CD36 in the liver (quantified using ImageJ); ( C ) Western blot analysis showing the expression of pAMPK, tAMPK, ABCA1, ABCG1, and CD36 in the aorta; ( D ) Relative protein expression levels of pAMPK, tAMPK, ABCA1, ABCG1, and CD36 in the aorta (quantified using ImageJ). Results are expressed as the means ± SEMs of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Membranes were blocked using 5% bovine serum albumin at room temperature for 1 h, followed by overnight incubation at 4 °C with primary antibodies targeting CD36 (PA1-16813, 1:1000, Thermo Fisher Scientific), ABCA1 (NB400-105, 1:1000, Novus Biologicals, Littleton, CO, USA), ABCG1 (NB400-132, 1:1000, Novus Biologicals), AMPK (2532, 1:1000, Cell Signaling Technology), phosphorylated AMPK (pAMPK, 2535, 1:1000, Cell Signaling Technology), and β-actin (ab8227, 1:10,000, Abcam, Cambridge, UK).

Techniques: Activity Assay, Expressing, Western Blot

Journal: Food Chemistry: Molecular Sciences

Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets

doi: 10.1016/j.fochms.2025.100253

Figure Lengend Snippet: Sequences of primers for quantitative real-time PCR.

Article Snippet: After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech).

Techniques:

Journal: Food Chemistry: Molecular Sciences

Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets

doi: 10.1016/j.fochms.2025.100253

Figure Lengend Snippet: Hepatic mRNA levels and protein expressions of CD36 in growing and adult rats. (A) Hepatic mRNA levels of CD36; (B) Hepatic protein expressions of CD36. Values are the means ± SEM (n = 6). Each measurement performed in triplicate as technical replicates. Bars marked with * are significantly different between CAS-G and RP-G under normal dietary condition ( P < 0.05). Bars marked with ** are significantly different between CAS-A and RP-An under normal dietary condition ( P < 0.05). Bars marked with # are significantly different between CAS-G and RP-G under oil-enriched dietary condition ( P < 0.05). Bars marked with ## are significantly different between CAS-A and RP-An under oil-enriched dietary condition ( P < 0.05). CD36, cluster determinant 36.

Article Snippet: After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech).

Techniques: